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one component substrate  (Bethyl)


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    Structured Review

    Bethyl one component substrate
    One Component Substrate, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/one+component+substrate/TMB+One+Component+Substrate/10__3390_slash_vetsci13040343-91-17-19
    Average 94 stars, based on 78 article reviews
    one component substrate - by Bioz Stars, 2026-09
    94/100 stars

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    other:

    Article Title: Characterization of a copper transporter 1 from <i>Dermanyssus gallinae</i> as a vaccine antigen
    Article Snippet: Finally, the wells were washed five https://doi.org/10.1017/S0031182021001608 Published online by Cambridge University Press times with PBS-T and reacted with the TMB one-component substrate (Bethyl Laboratories, Montgomery, TX, USA) for 20 min at room temperature in the dark.

    Article Title: Characterization of a Novel Cysteine Protease Inhibitor from Poultry Red Mites: Potential Vaccine for Chickens.
    Article Snippet: Finally, the wells were washed five times with PBS-T and allowed to react with the TMB one-component substrate (Bethyl Laboratories, Montgomery, TX, USA) for 20 min at 25 ◦C, in the dark.

    Article Title: Characterization of a copper transporter 1 from Dermanyssus gallinae as a vaccine antigen
    Article Snippet: Finally, the wells were washed five times with PBS-T and reacted with the TMB one-component substrate (Bethyl Laboratories, Montgomery, TX, USA) for 20 min at room temperature in the dark.

    Article Title: Characterization of a Novel Cysteine Protease Inhibitor from Poultry Red Mites: Potential Vaccine for Chickens
    Article Snippet: Finally, the wells were washed five times with PBS-T and allowed to react with the TMB one-component substrate (Bethyl Laboratories, Montgomery, TX, USA) for 20 min at 25 °C, in the dark.

    Binding Assay:

    Article Title: In Vitro Characterization of an Equinized Anti-PD-L1 Antibody for Cancer Immunotherapy in Horses
    Article Snippet: .. The binding of equine PD-L1 to PD-1 was visualized using NeutrAvidinhorseradish peroxidase (Thermo Fisher Scientific) and TMB One-Component Substrate (Bethyl Laboratories, Montgomery, TX, USA). ..



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    CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
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    CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
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    CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
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    Image Search Results


    CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off with PBST. Captured biotinylated mTNFα was detected with streptavidin-HRP and TMB substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.

    Journal: Frontiers in Immunology

    Article Title: Immunization with detoxified TNFα elicits neutralizing antibodies and ameliorates inflammatory shock and autoimmune arthritis in mice

    doi: 10.3389/fimmu.2026.1801223

    Figure Lengend Snippet: CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off with PBST. Captured biotinylated mTNFα was detected with streptavidin-HRP and TMB substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.

    Article Snippet: After washing with PBST, TMB substrate (Surmodics, Cat# TMBW-1000-01) was added.

    Techniques: Blocking Assay, Binding Assay, Vaccines, Enzyme-linked Immunosorbent Assay, Inhibition, Incubation